Culture Conditions Affect the Category and Production of Ubiquinones in a Recombinant Escherichia Coli with an Exogenous Decaprenyl Diphosphate Synthase
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چکیده
منابع مشابه
Optimization of Cloning Conditions for high-level Production of Recombinant Mouse Interleukin-2 in Escherichia coli
Backgrounds and objectives: Interleukin 2 (IL-2) secreted by activated CD4+ T cells has been known as a major mediator in both adaptive and native immune system due to a board range of effects on different cells in the immunity system (1-6). Methods: cDNA synthesis was performed using gene- specific primers designed by Gene Runner software after RNA extraction of mouse splenocytes. PCR pro...
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چکیده ندارد.
15 صفحه اولRv0989c encodes a novel (E)-geranyl diphosphate synthase facilitating decaprenyl diphosphate biosynthesis in Mycobacterium tuberculosis.
Mycobacterium tuberculosis (Mtb) has a highly complex cell wall, which is required for both bacterial survival and infection. Cell wall biosynthesis is dependent on decaprenyl diphosphate as a glyco-carrier, which is hence an essential metabolite in this pathogen. Previous biochemical studies indicated (E)-geranyl diphosphate (GPP) is required for the synthesis of decaprenyl diphosphate. Here w...
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Aminolevulinic acid (ALA) is formed by the enzyme ALA synthase (hemA gene). Then ALA is converted to Porphobilinogen (PBG) by the ALA dehydratase (hemB gene). For the overproduction of ALA, we used an Escherichia coli BL21(DE3) containing a hemA gene from Bradyrhzobium japonicum, which was created in our previous work. The effects of pH on the ALA synthase and ALA dehydratase were investigated....
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ژورنال
عنوان ژورنال: Advance Journal of Food Science and Technology
سال: 2013
ISSN: 2042-4868,2042-4876
DOI: 10.19026/ajfst.5.3080